Pricing

Service OUHSC Non-Academic
Add primer and Capillary Sequence
Primer added by facility personnel to <96 samples submitted in tubes or plates
$6.50/reaction   $16.50/rxn
Capillary sequencing of pre-mixed template and primer
Customer adds primer to template before submission
$6.00/reaction $14.25/reaction
Large Scale Custom DNA Sequencing
For submissions of full 96-well plates
$480.00/plate  $494.00/plate
Qiagen prep of cell pellet
Does not include the cost of sequencing
$2.75/pellet $6.63/reaction
Qiagen PCR clean up
Does not include the cost of sequencing
$5.50/cleanup  $9.38/cleanup
Small-Scale Fragment Analysis (microsatellite, AFLP, TRFLP and SNP)
Rate applies to submissions of less than 96 samples at a time of fluorescently labeled products for size or allele determination using the ABI 3730xl.  Fragment lengths can range from 35bp to 600bp for microsatellite samples and 15bp to 120bp for SNP samples.
*Please contact us directly if you require facility personnel to perform amplification with fluorescent primers as pricing will be affected.
$5.00/sample $8.88/reaction
High Throughput Fragment Analysis (microsatellite, AFLP, TRFLP and SNP)
Rate applies to submissions of 96 samples at a time of fluorescently labeled PCR products for size or allele determination using the ABI 3730xl.  Fragment lengths can range from 35bp to 600bp for microsatellite samples and 15bp to 120bp for SNP samples.
*Please contact us directly if you require facility personnel to perform amplification with fluorescent primers as pricing will be affected.
$3.00/sample $6.80/reaction

Illumina MiSeq sequencing of DNA and RNA samples

Service OUHSC Non-Academic
DNA Library Building
Nextera
$200.00/sample $304.00/sample
RNA Library Building
TruSeq
$300.00/sample $476.00/sample
Small RNA Library Building
TruSeq
$350.00/sample $484.00/sample
16S Metagenomics Library Building $300.00/(1-10) samples $750.00/(1-10) samples

ChIP DNA Library Building

$250.00/sample $484.00/sample
Cancer Panel
TruSeq Amplicon
Inquire  Inquire
50 Cycle MiSeq Run $1015.00/run $1081.00/run
300 Cycle MiSeq Run $1300.00/run $1380.00/run
500 Cyle MiSeq Run $1465.00/run $1554.00/run
600 Cycle MiSeq Run $1950.00/run $2045.00/run
Analysis  $300.00/run $350.00/run

Sample Clean-up and Quantitation

Service OUHSC Non-Academic
SAP/ExoI treatment
Only used when PCRs are submitted to the Core to set up and process.
$3.00/reaction $5.60/reaction
Nanodrop: RNA and DNA Quantitation  (up to 10 samples)
The Nanodrop uses undiluted sample in a minimum volume of 1µl and a maximum volume of 2µl which can be recovered after analysis.  Sensitivity range is from 5ng to 3700ng, making the Nanodrop useful for determining concentration of samples that may be limited in volume or that may be too dilute for accurate reading on other platforms.
$3.00 $5.70
Nanodrop: RNA and DNA Quantitation  (11-20 samples)
The Nanodrop uses undiluted sample in a minimum volume of 1µl and a maximum volume of 2µl which can be recovered after analysis.  Sensitivity range is from 5ng to 3700ng, making the Nanodrop useful for determining concentration of samples that may be limited in volume or that may be too dilute for accurate reading on other platforms.
$5.00 $7.80
Bioanalyzer: Total RNA, mRNA, DNA, and Protein Analysis (1-12 samples/run)
To determine quality of sample prior to use in downstream applications such as microarrays, real time PCR, sequencing, and binding assays.
$45.00/run $55.00/run
Bioanalyzer: small RNA AnalysisSmall RNA chips hold 11 samples. $71.00/run $61.00/run
Bioanalyzer: HS DNA for analysis of DNA samples of lower concentration (5-500pg/ul).  The chip holds 11 samples. $75.00/run  $85.00/run

Other Services

Service OUHSC Non-Academic
High throughput PCR
96-well PCR (25µl reactions) using the same primer pair for each sample.  Cost includes use of any of the facility provided universal primers listed on the sequencing section of our webpage.
*If custom primers are used then they must be added by the customer prior to cycling.  DNA template must be provided in 96- or 384- well format.
$2.00/sample (min. 48 samples) $6.50/sample (min. 48 samples)