CRACKING GEL BUFFER
1. For 50ml, add the following to a 50ml sterile conical tube:
- 2.5ml 1N NaOH
- 2.5ml 10% SDS
- 0.5ml 0.5M EDTA
- 25mg (or a few sprinkles to taste) Bromo-phenol blue
- 4g Sucrose
2. q.s. to 50ml using milliQ water.
3. Mix well by vortexing.
4. Can be stored at room temperature.