Cracking Gel Buffer

CRACKING GEL BUFFER

1.  For 50ml, add the following to a 50ml sterile conical tube:

  • 2.5ml   1N NaOH
  • 2.5ml   10% SDS
  • 0.5ml    0.5M EDTA
  • 25mg (or a few sprinkles to taste)  Bromo-phenol blue
  • 4g         Sucrose

2. q.s. to 50ml using milliQ water.

3. Mix well by vortexing.

4. Can be stored at room temperature.