Illumina MiSeq Next-Generation Sequencer

The Illumina MiSeq next generation sequencer is now in the Genomics division of the OUHSC core facilities! The MiSeq is identical to other Illumina sequencers like the HiSeq but costs and turnaround time are much less since the MiSeq is a single lane machine.  Each paired-end run on the MiSeq results in a single sequencing lane yielding  7.5-8.5 Gb of sequence data (500 cycle paired end run).  In addition, samples can be run individually or multiplexed using Illumina indexing chemistry which can further reduce per sample run costs. Cost estimates are given below for library construction, sequencing, and  bioinformatic analysis.

Applications Offered:

  • small genome resequencing
  • de novo sequencing and assembly of clinical isolates and other unknowns
  • small RNA profiling
  • whole transcriptome analysis
  • microbial community studies using multiplexed 16s amplicon pools
  • TruSeq amplicon Cancer panels (48 gene validated panel)
  • ChIP-Seq

Sample Submission Instructions

DNA Samples

Submit no less than 100 ng of purified, RNAse treated, genomic (prokaryotic or viral) DNA at a concentration of at least 20 ng/ul in nuclease-free water.

Whole Transcriptome Samples

Submit no less than 1 ug of high quality Total RNA at a concentration of at least 100 ng/ul in nuclease-free water. If you are unable to obtain this amount please contact us before submission.

Small RNA Samples

Total RNA: Please submit no less than 1 ug of high quality Total RNA at a concentration of at least 100 ng/ul in nuclease-free water. If you are unable to obtain this amount please contact us before submission.

Enriched Small RNA: The minimum amount that can be used for library constuction is 100 ng of enriched Small RNA.  Illumina’s protocol has been optimized for 500ng -1ug of enriched sRNA, and higher amounts of starting material typically increase quality of the resulting library.  If you are unable to obtain these higher amounts, please contact us before submission so that we can discuss expected results for this type of sample.

16s Metagenomic Samples

Please submit no less than 20ng of purified Microbial Genomic DNA at a concentration of at least 5 ng/ul in nuclease-free water. Samples are processed in batches of 10.
 

ChIP DNA Samples

Please submit 20-50 ng of ChIP DNA. If you are unable to obtain this amount please contact us before submission.

Online Submission Instructions

Request services by logging onto the OUHSC Core site via our iLab Solutions portal:  https://ouhsc.corefacilities.org/account/login

If you are an internal user, log in using your OUHSC username and password.   If you are an external user and do not already have an iLabs account, please register for an account.  Once you have logged in, please request the service of your choice.  If you have any questions, please email us at microgen_support@ouhsc.edu.

Current Pricing

Pricing (Current OUHSC Prices)

Library Building

Library Type Input Internal 
Pricing
External Pricing
DNA (Nextera) genomic DNA $200.00/sample $304.00/sample
RNA (TruSeq) Total RNA $300.00/sample $476.00/sample
Small RNA (TruSeq) Purified Small RNA, Total RNA $350.00/sample $484/sample
ChIP-Seq (TruSeq) IP DNA fragments $250.00/sample $353.20/sample
16S rRNA (TruSeq) Microbial DNA $300/(1-10) samples $750/(1-10) samples
Human genomic DNA, FFPE DNA Inquire for pricing    

Sequencing

Read Length (BP) Output Internal Pricing   External Pricing 
2 x 300 (600 Cycles) 14-15Gb $1950.00/run $2045.00/run
2 x 250 (500 Cycles) 7.5 - 8.5 Gb $1465.00/run $1554.00/run
2 x 150 (300 Cycles) 4.5 - 5.1 Gb $1300.00/run $1380.00/run
1 x 50 (50 Cycles) 750 - 850 Mb $1015.00/run $1081.00/run

Other

Service Internal Pricing  External Pricing 
Primary Analysis/Data Handling & Storage $300.00/run $350.00/run