BD FACSJazz

The FACSJazz cell sorter  installed in a specifically designed biological safety cabinet (BSC) allowing us to sort BSL2 samples. The system has 3 laser excitation (405, 488, and 647 nm) with the ability to measure up to 6 fluorescent parameters, 2 light scatter detectors, time, plus Pulse Processing for width and area measurements. The sensitivity is better than 125 MESF-FITC and 125 MESF-PE with less than 1 correlation error per 10^8 events.  The system has a fixed 100 um nozzle making it very stable and with less shear stress on live cells.  Sorting at a fixed 27 psi  pressure at 39 kHz with an average threshold of 10,000 events per second, a two-way sort achieved a purity of 98% and yield of >80% of Poisson’s expected yield for both populations. Index sorting is also available allowing the review of the complete flow phenotype of every cell sorted in to a multi-position sort device, such as a 96-well tray.  

The BD FACSJazz supports two-way sorting and Variety of different collection formats (with temperature control water bath) which include:

  • 5-ml tubes
  • 15-ml tubes
  • 96- and 384- well plates
  • Petri dishes
  • Terasaki plates
  • Custom collection devices

 Suggested Fluorophores with the laser and filter set configuration:

Laser Filter Fluorophore
488 530/30 Fitc, Alexa488, GFP, YFP
  585/29 PE, Cy3, or MT Orange
  670 LP PerCP, PerCP-Cy5.5, PE-Cy5.5, or PE-Cy7
640 660/20 APC, Alexa647 or MT deep red
  750 LP APC-Cy7 or H7
405 450/50 DAPI, Hoescht, BV421, HV450 or BFP

*** Note All Users are required to fill out a intake form when scheduling on iLabs ***

Rates OUHSC Non-Academic
Cell Sorting with assistance $85.00/ hour $118.89/ hour
Cancellation Charges (less than 24-hour notice) $35.00  

CALENDAR

For online scheduling navigate to iLabs for the core login page: https://ouhsc.corefacilities.org/

Minimum appointment time is 30 minutes and anything longer than 4 hours needs approval.

Any problems with iLab calendar read the help manuals below:

WHAT YOU NEED TO BRING

  • Cells should be at a concentration of 5x106 to 10x106/ml in a 12x75mm Falcon polypropylene tube
  • Unstained, Isotype and single color controls 
  • Lab coat

SAFE OPERATING PRACTICES (read before using equipment)

SOP for FACSJazz

Links