Nikon TE2000-E

NikonTE2000-EThe Nikon TE2000-E is an inverted epi-fluorescent microscoped with X-Y-Z control stage and environmental temperature control with a CO2 chamber.  The high precision motorized focus and vibration free optical path changeover mechanism that facilitates fast image capture in 3D.

Latest software update: Scan Slide allows us to quickly scan with a low power objective for image stitching a large area and then selecting regions of interest to be imaged with a higher magnification.

CONFIGURATION OF THE NIKON TE2000-E

  • X-Cite 120W lamp for fluorescence light source
  • Lamda 10-2 excitation and emission filter wheels
  • ASI motorized X-Y stage
  • Environmental chamber with CO2 control
  • Photometrics Cool Snap ES monochrome digital camera
  • Retiga ATI color digital camera

 Objectives:

  • 10x Plan Fluor NA 0.3 dry
  • 20x Plan Fluor NA 0.45 dry
  • 40x Plan Fluor NA 0.60 dry
  • 60x Plan Fluor NA 1.4 oil immersion
  • 100x Plan Fluor NA 1.4 oil immersion
  • Metamorph Software 

CALENDAR

For online scheduling navigate to iLabs for the core login page: https://ouhsc.corefacilities.org/

Minimum appointment time is 15 minutes and anything longer than 4 hours needs approval.

Any problems with iLab calendar read the help manuals below:

SAFE OPERATING PRACTICES  (read before using equipment)

SOP for Nikon TE2000                                                          

Rates   OUHSC  Non-Academic
With operator assistance $40.00/ hour $71.30/ hour
Without operator assistance (must be trained) $15.00/ hour $15.86/ hour
Cancellation Charges (less than 24-hour notice) $35.00  

LINKS

NIH Image - Free image processing and analysis program for Macintosh and Microsoft Windows 95 & NT.
Invitrogen SpectraViewer - For help in choosing fluorochomes.

MetaMorph software manual