The Stratedigm S1200Ex flow cytometer platform is a complete cellular analysis system consisting of a flow cytometer with 3 lasers and 13 colors, with scalability of adding up to 4 lasers capable of measuring 18 colors simultaneously, the CellCapTure acquisition and analysis software, A600 plate loader, and workstation.
The high-throughput temperature controlled A600 plate loader system has also been added to our Stratedigm system. The loader has the ability of sampling from various plate and tube formats: 96 and 384 well plates, eppendorph tubes, bullet tubes and 12x75mm tubes. The A600 plate loader is temperature controlled and can be used to add reagents and/or set incubation times for kinetic studies. With the 6-probe sampler, configurations can be defined using variable mixing cycles and mixing volumes, which is essential for experimental design and faster throughput. The sample volumes for adding reagents or injecting samples into the cytometer can be defined to as little as 2ul or as much as 500ul. Data for a 96-well plate can be collected under 25 minutes.
|Analysis without assistance
|Analysis with assistance
|Cancellation Charges (less than 24-hour notice)
For online scheduling navigate to iLabs for the core login page: https://ouhsc.corefacilities.org/
Minimum appointment time is 10 minutes and anything longer than 4 hours needs approval.
Any problems with iLab calendar read the help manuals below:
WHAT YOU NEED TO BRING
- Cells should be at a concentration of 5x105 to 5x106/ml
- Samples can be in 96-well plates, 12x75ml tubes, eppendorph tubes, or 1.2ml cluster tubes
- Unstained, Isotype and single color controls
- Lab coat
SAFE OPERATING PRACTICES (read before using equipment)
SOP for Stratedigm