MALDI-TOF

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) is a powerful tool for the analysis of biomolecules such as proteins, peptides, sugars and DNA. The principle of MALDI is based on the photo-volatilization of a sample embedded in a UV-absorbing matrix. Briefly, the sample with small organic compound (matrix) is deposited on a conductive sample support to form a crystalline structure. The co-crystals are then irradiated with a laser beam and a particle cloud is generated. MALDI-TOF can analyze a wide range of masses, but is best used for measurement of >1 kDa. Typically, samples will contain at least 10-100 pmol/μl and at least 10 μl. Samples should be free of buffer salts, detergents, acids, and bases. Please note on the sample submission sheet if your sample contains any of these items and their concentrations.

Clean-up can be performed by facility personnel for an additional fee. For any help on your samples, please contact Lmbcr_help@ouhsc.edu.

For online submission navigate to iLabs for the core login page: https://ouhsc.corefacilities.org/

Any problems with iLab read the help manuals below: