FACSAria Fusion

The Aria Fusion is equipped with 4 lasers, UV (15mW @355nm), Blue (50mW @488nm), Red (100mW @633nm), and Green (50mW @561nm). It is capable of measuring up to 11 fluorescescent parameters plus the forward and side scatter signals.  The instrument also has four nozzle sizes (70um, 85um, 100um, 130um) and is capable of several sort collection options: 1.5 ml eppendorphs, 5 ml, and 15 ml tubes, plus index sorting into 96 or 384-well plates.  

This system supports a variety of different collection tube formats (with temperature control water bath) which include:  

  • Two-way sorting: 12 x 75-mm tubes or 15-mL tubes
  • Four-way sorting: 1.5-mL eppendorph tubes or 12 x 75-mm tubes 

 

 Suggested Fluorophores with the laser and filter set configuration:

Screen Shot 2018-08-02 at 4.27.43 PM

*** Note All Users are required to fill out a intake form when scheduling on iLabs ***

Rates OUHSC Non-Academic
Cell Sorting with assistance $90.00/ hour $123.89/ hour
Cancellation Charges (less than 24-hour notice) $35.00  
 

CALENDAR

For online scheduling navigate to iLabs for the core login page: https://ouhsc.corefacilities.org/ 

Minimum appointment time is 30 minutes and anything longer than 4 hours needs approval.

Any problems with iLab calendar read the help manuals below:

WHAT YOU NEED TO BRING

  • Cells should be at a concentration of 5x106 to 10x106/ml
  • Samples can be in a 12x75ml tube or 15ml conical tube
  • Unstained, Isotype and single color controls 
  • Lab coat

 

SAFE OPERATING PRACTICES (read before using equipment)

Links