Laboratory of Biomolecular Structure and Function

The Laboratory of Biomolecular Structure and Function (LBSF) was created in 2015 to serve scientists of Oklahoma City, in particular OUHSC and OMRF, that are interested in structural aspects of their macromolecular targets.The LBSF is part of the OUHSC Vice President Research Core Facilities. LBSF was created on the basis of the Macromolecular Crystallography Laboratory of the Department of Biochemistry and Molecular Biology. This earlier lab was created in 1998 and became a core facility for the  Oklahoma Center of Biomedical Research Excellence (CoBRE) in Structural Biology (OCSB). The LBSF is one of two sites that make up the Biomolecular Structure Core of the OCSB. The other site is the Macromolecular Crystallography Laboratory (MCL) at OU-Norman. Users of the LBSF also have access to the MCL which a complementary set of instruments. 

The LBSF is equipped with modern crystallographic equipment and computers: A 2015 Rigaku 007 generator, R-axis IV and Mar345 image plate detectors, Osmic mirrors on both ports, Rigaku and Oxford Systems crystal cooling systems. The R-AXIS IV has an optional helium path for working with large unit cells. 

D-TrekXDSMosflm and HKL2000 image processing software are available for data processing, Any of the PhenixCCP4CNS program suites can be used for structure determination and refinement, while COOTChimera and Molmol are offered for graphical representation of the results. 

The LBSF has a 2014 Wyatt plate reading dynamics light scattering instrument that enables high-throughput data collection with no user intervention for evaluation of the oligomerization states of  proteins. Monodisperse solutions with 5-20 mg/ml of protein are required for crystallization experiments, small angle x-ray scattering experiments, and cryo electron microscropy (cryo-EM) experiments. 

The LBSF has a 2019 Leica cryo-plunger for the preparation of cryo-grids for cryo-EM work. 

200 microliters of 95% pure protein sample at a concentration of 5-20 mg/ml are needed to set up ten 96-well trays with the 2018 Art Robbins Gryphon LCP crystallization robot. The robot can also set up crystallization experiments in the lipidic cubic phase for the crystallization of membrane proteins.

Services and Fees, Internal and [External] 

  • Molecular cloning, 22.07/h [$43.96/h]
  • Protein over-expression, purification, or both; 22.07 [$43.96]
  • 96-well crystallization tray with robot for finding initial lead,  $29.43/tray [$40.23/tray]
  • 24-well crystallization tray for crystal size optimization, $34.61/tray [$67.32/tray]
  • 15-well crystallization tray for crystal size optimization, $34.04/tray [$68.81/tray]
  • Crystal handling services, $22.07/h [$43.96/h]
    • evaluating results of crystallization experiments
    • screening of crystals for diffraction at room temperature
    • screening of crystals for cryo-conditions
    • local X-ray diffraction data collection
  • X-ray computation services, 22.07/h [$43.96/h]
    • Remote data collection at synchrotron radiation lab
    • X-ray diffraction data processing
    • Structure determination
    • Structure refinement
    • Structure validation analysis
    • Figure making
  • Molecular modeling services by Dr. Mather, $75.00/h [$75.00/h]

Main Users

Staff

Simon Terzyan, PhD – Staff Scientist (BRC 406 and 472)

Tim Mather, PhD - Molecular Modeling Facility (BRC 238)


Location

The Laboratory is located in the Biomedical Research Center Building on the University of Oklahoma Health Sciences Center. The 

975 NE 10th Street Biomedical Research Center Building Oklahoma City, Oklahoma 73104 (405) 271-8300 Email Contact

Download a Campus Map

 

Useful websites

Upcoming events

A new 5-week graduate course on Structural Biology (course director: Dr. Pioszak) in the first session, Spring Semester 2020. 

A new 5-week graduate course on Biochemical and Biophysical Instrumentation (course director Dr. Lin) included two lecture on X-ray diffraction experiments, Spring Semester 2020.


2020 SSRL/LCLS User Meeting will be virtual this year. Student poster presenters will have their registration fee waived. For more information.