Laboratory of Biomolecular Structure and Function

The Laboratory of Biomolecular Structure and Function (LBSF) was created in 2015 to serve scientists of Oklahoma City, in particular OUHSC and OMRF, that are interested in structural aspects of their macromolecular targets. It is one of four core facilities in the Oklahoma Center of Biomedical Research Excellence (CoBRE) in Structural Biology (OCSB)and is part of the OUHSC Vice President Research Core Facilities. LBSF was created on the basis of the Macromolecular Crystallography Laboratory of the Biochemistry and Molecular Biology Department of OUHSC created in 1998, which was converted to core facility of OCSB in 2012.

The laboratory itself is equipped with modern crystallographic equipment and computers: A 2015 Rigaku 007 generator, R_axis IV and Mar345 image plate detectors, Osmic mirrors on both ports, Rigaku and Oxford Systems crystal cooling systems. The R-AXIS IV has an optional helium path for working with large unit cells. D-TrekXDSMosflm and HKL2000 image processing software are available for data processing, Any of the PhenixCCP4CNS program suites can be used for structure determination and refinement, while COOTChimera and Molmol are offered for graphical representation of the results. A 2014 Wyatt plate reading dynamics light scattering instrument that enables high-throughput data collection with no user intervention for evaluation of the oligomerization states of  proteins. A 2018 Art Robbins Gryphon LCP crystallization robot can set up crystallization plates for soluble proteins and for membrane proteins using the lipidic cubic phase.

Services

Laboratory provides following services:

  • Advice on protein construct design, expression, and purification
  • Help with protein purification (BRC 406)
  • Dynamic light scattering data collection and analysis
  • Crystallization set up with crystallization robot for high throughput screening
  • Crystallization set up by robot or manually for crystal size optimization experiments
  • X-ray crystal screening and quality evaluation
  • Optimization of cryo protectant solutions
  • On-site X-ray data collection, processing and evaluation
  • Remote X-ray data collection at synchrotron radiation at SSRL
  • Structure determination, structure refinement, structure analysis
  • Design of mutant proteins based on structural information
  • Preparation of figures for grant applications and publication.
  • Molecular modeling and structure-based drug design (Dr. Tim Mather)
  • Coming in late 2019, preparation of cryo electron microscopy grids with cryo plunger 

Main Users

Staff

Simon Terzyan, PhD – Staff Scientist (BRC 472)

Tim Mather, PhD - Molecular Modeling Facility (BRC 238)

Fees

$5.00/hour for X-ray diffraction data collection system usage

$50.00/hour for assistance of personal.

$50.00 for 96-well crystallization tray set up with robot.

Location

The Laboratory is located in the Biomedical Research Center Building on the University of Oklahoma Health Sciences Center. The 

975 NE 10th Street Biomedical Research Center Building Oklahoma City, Oklahoma 73104 (405) 271-8300 Email Contact

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Useful websites

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