TapeStation, Bioanalyzer, NanoDrop, and Qubit

TapeStation and Bioanalyzer:  

• Please submit a minimum of at least 5ul of each sample in a DNase/RNase free eppendorf tube.  We will check the OD of your sample before running it on a chip.  It is often best to submit no more that 5ul to eliminate the need to pick up remaining sample.
• Dilute DNA or RNA to the following recommended sample concentrations:

DNA: 0.1-50ng/ul (size range 35-1000bp)

HS DNA: (high sensitivity) 5-1000pg/ul (size range 35-1000bp)

HS DNA 5000: (high sensitivity) 10-1000pg/ul (size range 100-5000bp)

Genomic DNA:  10-100ng/ul (size range 200-60,000bp)

          RNA: (Total RNA and mRNA) 25-500ng/ul

          HS RNA: (high sensitivity) 100-10,000pg/ul

          Small RNA on Bioanalyzer only: 50-2000pg/ul (size 

                                  range 6-150nt) *1-11 samples per chip

• Samples will be processed as quickly as possible, usually within 2 work days.  You will receive a PDF containing your data.
• The TapeStation is per sample. The Bioanalyzer chips are one use only (empty wells cannot be rerun) so they are 1-11 samples.  
• Place DNA samples in our -20 freezer or RNA samples in our -80 freezer.  Both are located in BRC 1106.

NanoDrop:

• Please submit 3ul of each sample in properly labeled tubes or plates.  Please do no use flat-bottom plates.
• Samples will be processed as quickly as possible, usually within 2 work days.
• Place DNA samples in our -20 freezer or RNA samples in our -80 freezer.  Both are located in BRC 1106.

Qubit:

• Please submit a minimum of at least 5ul of each sample in a DNase/RNase free eppendorf tube. 
• This service does not have it's own request form in iLab.  Please contact the Genomics group before requesting Qubit readings.
• genomics_support@ouhsc.edu
• phone: (405) 271-2337

Shipping Address:

Attn: Genomics Core

975 NE 10th St. BRC Room 1106

Oklahoma City, OK 73104

Service requests should be made through the OUHSC Core iLab Solutions portal: https://ouhsc.corefacilities.org/account/login  Once logged in, navigate to the “Request Services” tab and select the service for your project. 

If you do not have an iLab account, please visit the log-in page listed above and click on the “Register” button in the upper right corner.  For internal users, register using your OUHSC username and Password.