The Nikon TE2000-E is an inverted epi-fluorescent microscoped with X-Y-Z control stage and environmental temperature control with a CO2 chamber. The high precision motorized focus and vibration free optical path changeover mechanism that facilitates fast image capture in 3D.
CURRENT CONFIGURATION
- X-Cite 120W lamp for fluorescence light source
- Lamda 10-2 excitation and emission filter wheels
- ASI motorized X-Y stage
- Environmental chamber with CO2 control
- Photometrics Cool Snap ES monochrome digital camera
- Retiga ATI color digital camera
Objectives
- 10x Plan Fluor NA 0.3 dry
- 20x Plan Fluor NA 0.45 dry
- 40x Plan Fluor NA 0.60 dry
- 60x Plan Fluor NA 1.4 oil immersion
- 100x Plan Fluor NA 1.4 oil immersion
- Metamorph Software
| Rates |
OUHSC |
External |
Industry |
| Services with operator assistance |
$40/ hour |
$80/ hour |
$120/ hour |
| Services without operator assistance (must be trained) |
$20/ hour |
$40/ hour |
$60/ hour |
| Cancellation Charges (less than 24-hour notice) |
$35.00 |
|
|
CALENDAR
For online scheduling navigate to iLabs for the core login page: https://ouhsc.corefacilities.org/
Minimum appointment time is 15 minutes and anything longer than 4 hours needs approval.
Any problems with iLab calendar read the help manuals below:
SAFE OPERATING PRACTICES (read before using equipment)
SOP for Nikon TE2000
LINKS
NIH Image - Free image processing and analysis program for Macintosh and Microsoft Windows 95 & NT.
Invitrogen SpectraViewer- For help in choosing fluorochomes.
MetaMorph software manual