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Illumina Next Generation Sequencing

The Genomics Core has a new NextSeq 2000 sequencer from Illumina allowing for sequencing of all library types.

Cost estimates are given below for library construction, sequencing, and  bioinformatic analysis.

Applications Offered:

  • RNA-seq 
  • 16S-seq
  • DNA-seq
  • ChIP-Seq
  • And more: Please Inquire

Sample Submission Instructions

DNA Samples

Submit no less than 100 ng of purified, RNAse treated, genomic (prokaryotic or viral) DNA at a concentration of at least 20 ng/ul in nuclease-free water.

RNA-seq Samples

Submit no less than 1 ug of high quality Total RNA at a concentration of at least 100 ng/ul in nuclease-free water. If you are unable to obtain this amount please contact us before submission.

16s Samples

Please submit no less than 20ng of purified Microbial Genomic DNA at a concentration of at least 5 ng/ul in nuclease-free water.  The DNA will be amplified with the standard 16S primers unless otherwise requested.  If you have performed the 16s PCR before submitting, please make sure to clean up the PCR and submit 10ul of the purified PCR product.
 

ChIP DNA Samples

Immunoprecipitation must be performed in the users lab.  Please submit 20-50 ng of ChIP DNA (200-800bp in size). If you are unable to obtain this amount please contact us before submission.

 

Other Library Types

Please inquire:  genomics@groups.ou.edu  or jenny-gipson@ouhsc.edu

 

On-line Submission Instructions

Request services by logging onto the OUHSC Core site via our iLab Solutions portal:  https://ouhsc.corefacilities.org/account/login

If you do not already have an account, please click on the "Register" button in the upper right corner.

If you are an internal user, log in using your OUHSC username and password.   Once you have logged in, please request the service of your choice.  If you have any questions, please email us at genomics@groups.ou.edu

 

Current Pricing

Library Building

Library Type Internal Pricing External Pricing Industry Pricing
DNA $100/sample $145/sample $210.25/sample
RNA-seq $80/sample $116/sample $168.20/sample
16S-seq  $30/sample $43.50/sample $63.08/sample
All other library types inquire inquire inquire
       

Library Sequencing

Library Type System/Read # Internal Pricing External Pricing Industry Pricing
DNA per M reads $3.50/M $5.08/M $7.37/M
RNA-seq per M reads $3.50/M $5.08/M $7.37/M
16S-seq 600 cycle kit  $1,900/run (100M reads) $2,755.00 $3,994.75
Other Library Types *can vary, please inquire
any libraries needing custom
sequencing primers or special
cycling kits may cost more		  as low as $3.50/M* $5.08/M $7.37/M