The Nikon TE2000-E is an inverted epi-fluorescent microscoped with X-Y-Z control stage and environmental temperature control with a CO2 chamber. The high precision motorized focus and vibration free optical path changeover mechanism that facilitates fast image capture in 3D.The available offline IDEAS software (ver 6.2) has pre-configured and optimized wizards for many common applications
CURRENT CONFIGURATION
- X-Cite 120W lamp for fluorescence light source
- Lamda 10-2 excitation and emission filter wheels
- ASI motorized X-Y stage
- Environmental chamber with CO2 control
- Photometrics Cool Snap ES monochrome digital camera
- Retiga ATI color digital camera
Objectives
- 10x Plan Fluor NA 0.3 dry
- 20x Plan Fluor NA 0.45 dry
- 40x Plan Fluor NA 0.60 dry
- 60x Plan Fluor NA 1.4 oil immersion
- 100x Plan Fluor NA 1.4 oil immersion
- Metamorph Software
Rates
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OUHSC
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External
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Industry
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Services with operator assistance
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$40/ hour
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$80/ hour
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$120/ hour
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Services without operator assistance (must be trained)
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$20/ hour
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$40/ hour
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$60/ hour
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Cancellation Charges (less than 24-hour notice)
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$35.00
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CALENDAR
For online scheduling navigate to iLabs for the core login page: https://ouhsc.corefacilities.org/
Minimum appointment time is 15 minutes and anything longer than 4 hours needs approval.
Any problems with iLab calendar read the help manuals below:
SAFE OPERATING PRACTICES (read before using equipment)
SOP for Nikon TE2000
LINKS
NIH Image - Free image processing and analysis program for Macintosh and Microsoft Windows 95 & NT.
Invitrogen SpectraViewer- For help in choosing fluorochomes.
MetaMorph software manual