Parent Page: Research Support id: 26996 Active Page: Confocal microscopy id: 29533


Leica SP8 confocal microscope

The Leica SP8 confocal microscope is equipped with two high sensitivity, low-noise hybrid detectors capable of photon counting and two conventional PMT detectors. Sample excitation can be accomplished by multiple lasers including a pulsed white light laser tunable from 470 to 670 nm that allows selection of the optimal excitation wavelength.  The LightGate function is a filter free removal of non-wanted background allowing gating of the detectors to avoid collecting signal during the laser pulse so that only pure emission signals are collected. The system also has conventional fixed laser excitation lines at 405 and 442nm.

Lasers and Detectors

  • White Light Laser system (470 to 670 nm) tunable in steps of 1 nm
  • UV / 405 nm laser
  • Violet / 442 nm laser
  • 4 channels of Confocal Detection
  • 2 Super Sensitive Spectral Hybrid detectors
  • 2 cooled PMT detectors


  • Field-of View Scanning system for imaging at speeds from 1 to 1800Hz
  • Scanning XY-stage for montage imaging
  • Stage top incubation system - 5% CO2,, temperature controlled, compatible with 35mm dish
  • Adaptive Focus Control
  • LightGate Imaging via pulsed WLL and HyD detectors helps remove reflected light as well as autofluorescence
  • Z-stack for 3-D rendering
  • Brightfield:
  • Transmission
  • Reflection
  • Fluorescence
  • Multi-color simultaneous acquition
  • Serial acquition
  • Time lapse
  • FRET
  • FRAP


  • 63x Plan APO 1.4 NA oil (140um working distance)
  • 40x HC APO 1.3 NA oil (240um working distance)
  • 40x HCX PL APO  0.85 NA dry (210um working distance)
  • 20x HC PL APO 0.75 IMM (660um working distance)
  • 10x HC PL APO 0.4 NA dry (2.63mm working distance)
Rates OUHSC External Industry
Services with operator assistance $70/ hour $147/ hour $210/ hour
Services without operator assistance (must be trained) $40/ hour $80/ hour $120/ hour
Cancellation Charges (less than 24-hour notice) $35.00    


For online scheduling navigate to iLabs for the core login page:

Minimum appointment time is 15 minutes and anything longer than 4 hours needs approval.

Any problems with iLab calendar read the help manuals below:

SAFE OPERATING PRACTICES (read before using equipment)

SOP for Leica SP8