The Laboratory of Biomolecular Structure and Function (LBSF) was created in 2015 to serve scientists of Oklahoma City, in particular OUHSC and OMRF, that are interested in structural aspects of their macromolecular targets.The LBSF is part of the OUHSC Vice President Research Core Facilities. LBSF was created on the basis of the Macromolecular Crystallography Laboratory of the Department of Biochemistry and Molecular Biology. This earlier lab was created in 1998 and became a core facility for the Oklahoma Center of Biomedical Research Excellence (CoBRE) in Structural Biology (OCSB). The LBSF is one of two sites that make up the Biomolecular Structure Core of the OCSB. The other site is the Macromolecular Crystallography Laboratory (MCL) at OU-Norman. Users of the LBSF also have access to the MCL which a complementary set of instruments.
The LBSF is equipped with modern crystallographic equipment and computers: A 2015 Rigaku 007 generator, R-axis IV and Mar345 image plate detectors, Osmic mirrors on both ports, Rigaku and Oxford Systems crystal cooling systems. The R-AXIS IV has an optional helium path for working with large unit cells.
D-Trek, XDS, Mosflm and HKL2000 image processing software are available for data processing, Any of the Phenix, CCP4, CNS program suites can be used for structure determination and refinement, while COOT, Chimera and Molmol are offered for graphical representation of the results.
The LBSF has a 2014 Wyatt plate reading dynamics light scattering instrument that enables high-throughput data collection with no user intervention for evaluation of the oligomerization states of proteins. Monodisperse solutions with 5-20 mg/ml of protein are required for crystallization experiments, small angle x-ray scattering experiments, and cryo electron microscropy (cryo-EM) experiments.
The LBSF has a 2019 Leica cryo-plunger for the preparation of cryo-grids for cryo-EM work.
200 microliters of 95% pure protein sample at a concentration of 5-20 mg/ml are needed to set up ten 96-well trays with the 2018 Art Robbins Gryphon LCP crystallization robot. The robot can also set up crystallization experiments in the lipidic cubic phase for the crystallization of membrane proteins.
Services and Fees, Internal and [External]
- Molecular cloning, 22.07/h [$43.96/h]
- Protein over-expression, purification, or both; 22.07 [$43.96]
- 96-well crystallization tray with robot for finding initial lead, $29.43/tray [$40.23/tray]
- 24-well crystallization tray for crystal size optimization, $34.61/tray [$67.32/tray]
- 15-well crystallization tray for crystal size optimization, $34.04/tray [$68.81/tray]
- Crystal handling services, $22.07/h [$43.96/h]
- evaluating results of crystallization experiments
- screening of crystals for diffraction at room temperature
- screening of crystals for cryo-conditions
- local X-ray diffraction data collection
- X-ray computation services, 22.07/h [$43.96/h]
- Remote data collection at synchrotron radiation lab
- X-ray diffraction data processing
- Structure determination
- Structure refinement
- Structure validation analysis
- Figure making
- Molecular modeling services by Dr. Mather, $75.00/h [$75.00/h]
- Blaine H. M. Mooers, PhD
- Marie Hanigan, PhD
- Augen Pioszak, PhD
- Franklin A. Hays, PhD
- Xin Zhang, MD, PhD
- Jie Wu, PhD
- Ralf Janknecht, PhD
- Wei-Qun Ding, PhD
- Jody Summers, PhD
- Jialing Lin, PhD
- Ian Dunn, MD
- Simon Terzyan, PhD – Staff Scientist (BRC 406 and 472)
- Tim Mather, PhD - Molecular Modeling Facility (BRC 238)
The Laboratory is located in the Biomedical Research Center Building on the University of Oklahoma Health Sciences Center. The 975 NE 10th Street Biomedical Research Center Building Oklahoma City, Oklahoma 73104 (405) 271-8300 Email Contact
Download a Campus Map
- Macromolecular Crystallopgrahy Laboratory, OU-Norman Campus
- SBGrid Consortium
- American Crystallography Association
- International Union of Crystallography
- HKL-2000 manual
- Rigaku’s homepage
- Mar345 Manual
- Crystal Clear manual (includes d*TREK)
- XDS Manual
- Phenix manual
- CCP4 homepage
- CNS manual
- SHELX homepage
- Graphics program COOT manual
- Biological Small Angle Scattering Links
- Molecular Graphics Links from the 'PyMOL' course
- PyMOL for molecular artwork; download site. First-year GPiBS students have the incentive version of PyMOL. We are still trying to get a site license for the University.
- Open source PyMOL for Windows, open source is functional but exporting movies takes longer.
- Open source PyMOL for Mac, the macports version installs works well.
- EasyPyMOL, get started for horizontal scripting in PyMOL
- pymolshortcuts, shortcuts for greater productivity in PyMOL
- pymolsnips, PyMOL snippet library for text editors
- pymolpysnips, PyMOL snippet library for Jupyter Notebooks
- UCSF Chimera, useful for making images and structure analysis
- Jmol, a molecular graphics program based on Java.
- Ligplot, a program for creation of ligand-protein interaction diagrams
- Ligplot manual
- More crystallographic links
- A 5-week graduate course on the Methods of Structural Biology (course director: Dr. Pioszak) in the first session, Spring Semester 2021.
- A 5-week graduate course on Machine Learning in BioMedicine (course director Dr. Franklin Hays) includes a lecture on the use of deep learning in structure-based drug design, third session, Spring Semester 2021.
- A 5-week graduate course on Biochemical and Biophysical Instrumentation (course director Dr. Lin) includes two lectures on X-ray diffraction experiments, third session, Spring Semester 2021.
- 2020 SSRL/LCLS User Meeting was virtual this year. The videos of the talks are available online. For more information.