Single Cell and Spatial Transcriptomics

Single Cell RNA-Seq

• cells should be live dead sorted, and a test sort should be run on your samples prior to submission date to make sure you are getting the number of cells and viability needed
• cells should be at 70% viable or greater
• a date for the experiment must be set beforehand so that all library building reagents are in the lab and time can be devoted to handling your samples
• please have cells ready by 1pm.  If you need to submit cells later than 1pm, please schedule that with the genomics personnel

• fresh-frozen tissue samples can be processed without the CytAssist
• all samples processed on the CytAssist must be FFPE samples and can be freshly sectioned or previously sectioned and even stained
• the CytAssist eliminates the need to score blocks in order to fit into the capture areas
• capture area sizes are either 4 sets of 6.5mm or 2 sets of 11mm
• FFPE samples need to be previously processed for RNA to determine RNA quality before the experiment.  FFPE quality must be >30% DV200.

Request services by logging onto the Institutional Research Core Facility site via our iLab Solutions portal:  https://ouhsc.corefacilities.org/account/login

If you do not already have an account, please click on the "Register" button in the upper right corner.

If you are an internal user, log in using your OUHSC username and password.   Once you have logged in, please request the service of your choice.  If you have any questions, please email us at genomics@groups.ou.edu

Single Cell Library Preparation (sequencing prices below)

Spatial Transcriptomics (sequencing prices below)

Sequencing