The Agilent 2100 Bioanalyzer is a microfluids-based platform replacing standard gel electrophoresis for RNA sample QC, DNA fragment analysis, and SDS-page electrophoresis for proteins.
The small volume allows you to conserve valuable sample and with a 30 minute run-time per chip you will be able to determine the quality and quantity of up to 12 total RNA samples and 11 small RNA samples. The analysis will include graphic visualization of ribosomal peaks (23S and 16S) or (28S and 18S), ribosomal ratio, RNA concentration, a calculated RIN (RNA Integrity Number) value, and a gel-like image RNA sample. Although the Bioanalyzer analysis will quantitate RNA samples, in our experience RNA readings are more accurate using the NanoDrop ND-1000 UV-Vis Spectrophotometer which we provide for each sample submitted. NanoDrop is also available as a separate service for both RNA, DNA, and protein quantitation.
The following is a partial list of applications for Agilent’s Lab-on-a-Chip® technology for RNA, DNA, and protein analysis. For a more complete list of applications to meet your research needs. At this time the Bioanalyzer 2100 in the core facility is not equipped with a flow cell needed for cell fluorescence analysis.
- Check RNA integrity for downstream applications
- Genomic and ribosomal contamination of mRNA samples
- Small RNA (6-150 nt) analysis of miRNA, siRNA, and oligonucleotides
- Sizing of T7-RNA transcripts
- PCR product purity and size
- Restriction digests
- Protein induction in cell lysates
- Protein purification
- Glycoprotein sizing