Illumina Next-Generation Sequencing

DNA Samples

Submit no less than 100 ng of purified, RNAse treated, genomic (prokaryotic or viral) DNA at a concentration of at least 20 ng/ul in nuclease-free water.

Whole Transcriptome Samples

Submit no less than 1 ug of high quality Total RNA at a concentration of at least 100 ng/ul in nuclease-free water. If you are unable to obtain this amount please contact us before submission.

Small RNA Samples

Total RNA: Please submit no less than 1 ug of high quality Total RNA at a concentration of at least 100 ng/ul in nuclease-free water. If you are unable to obtain this amount please contact us before submission.

Enriched Small RNA: The minimum amount that can be used for library constuction is 100 ng of enriched Small RNA.  Illumina’s protocol has been optimized for 500ng -1ug of enriched sRNA, and higher amounts of starting material typically increase quality of the resulting library.  If you are unable to obtain these higher amounts, please contact us before submission so that we can discuss expected results for this type of sample.

16s Metagenomic Samples

Please submit no less than 20ng of purified Microbial Genomic DNA at a concentration of at least 5 ng/ul in nuclease-free water. Samples are processed in batches of 10.  If you have performed the 16s PCR before submitting, please make sure to clean up the PCR and submit 10ul of the purified PCR product.
 

ChIP DNA Samples

Please submit 20-50 ng of ChIP DNA (200-800bp in size). If you are unable to obtain this amount please contact us before submission.

Request services by logging onto the OUHSC Core site via our iLab Solutions portal:  https://ouhsc.corefacilities.org/account/login

If you are an internal user, log in using your OUHSC username and password.   If you are an external user and do not already have an iLabs account, please register for an account.  Once you have logged in, please request the service of your choice.  If you have any questions, please email us at microgen_support@ouhsc.edu.

Pricing (Current OUHSC Prices)

Library Building

Sequencing

Other

 Once a MiSeq Run is complete, we will transfer the raw data to CLC Genomics Workbench (Qiagen) version 10.0.1 and newer for analysis

         The analysis will include aligning the data to your reference genome of choice or de novo assembly, and initial analysis will be performed.  Below are files that can be expected for each type of sample analysis.

DNA Samples:  Mapping files, Summary Report, Mapping Report, Variant Reports when possible, and Unmapped Reads upon request                                                       

Whole Transcriptome Samples: Gene Expression list, Differential Expression list; and initial PCA plot, Heat Map, and Venn Diagram images                           

Small RNA Samples: small RNA Report, small RNA Sample list, Comparison lists               

16S Samples:  OTU Report, Trim Report, OTU Table, Comparison Tables                         

ChIP Samples: Summary Report, Peaks, QC Report, Peak Shape Filter, Peak Shape Score, Peaks Annotated                       

             ** Many files are interactive in the CLC Genomics Workbench Software.  Customers are responsible for additional analysis and may schedule a time to use the CLC software.      

·      Once the analysis has been performed, the files will be exported and either emailed to the customer or backed up to an encrypted drive provided by either the customer or purchased through our Core (8GB - 2TB recommended for most projects).  The customer must also back up the raw data files (.fastq) and be responsible for keeping any files that might be needed in the future.  We will keep raw data files (.fastq) for up to 3 months but if analysis has to be repeated due to a customer not saving his or her data, a charge might be applied.