FACSJazz

The FACSJazz cell sorter  installed in a specifically designed biological safety cabinet (BSC) allowing us to sort BSL2 samples. The system has 3 laser excitation (405, 488, and 647 nm) with the ability to measure up to 6 fluorescent parameters, 2 light scatter detectors, time, plus Pulse Processing for width and area measurements. The sensitivity is better than 125 MESF-FITC and 125 MESF-PE with less than 1 correlation error per 10⁸ events.  The system has a fixed 100 um nozzle making it very stable and with less shear stress on live cells.  Sorting at a fixed 27 psi  pressure at 39 kHz with an average threshold of 10,000 events per second, a two-way sort achieved a purity of 98% and yield of >80% of Poisson’s expected yield for both populations. Index sorting is also available allowing the review of the complete flow phenotype of every cell sorted in to a multi-position sort device, such as a 96-well tray.  

The BD FACSJazz supports two-way sorting and Variety of different collection formats (with temperature control water bath) which include:

• 5-ml tubes
• 15-ml tubes
• 96- and 384- well plates
• Petri dishes
• Terasaki plates
• Custom collection devices

 Suggested Fluorophores with the laser and filter set configuration:

*** Note All Users are required to fill out a intake form when scheduling on iLabs ***

CALENDAR

For online scheduling navigate to iLabs for the core login page: https://ouhsc.corefacilities.org/

Minimum appointment time is 30 minutes and anything longer than 4 hours needs approval.

Any problems with iLab calendar read the help manuals below:

• Core Facilities Scheduling Core Customer Help Manual
• Core Facilities Core Customer Help Manual

WHAT YOU NEED TO BRING

• Cells should be at a concentration of 5x10⁶ to 10x10⁶/ml in a 12x75mm Falcon polypropylene tube
• Unstained, Isotype and single color controls 
• Lab coat

SAFE OPERATING PRACTICES (read before using equipment)

• SOP for FACSJazz

Links

• Sample prep for sorting
• FACSJazz Specifications
• FluoroFinder