Leica SP8 Confocal

SP8

 

 

Leica SP8 Confocal

Lasers and Detectors

  • White Light Laser system (470 to 670 nm) tunable in steps of 1 nm
  • UV / 405 nm laser
  • Violet / 442 nm laser
  • 4 channels of Confocal Detection
  • 2 Super Sensitive Spectral Hybrid detectors
  • 2 cooled PMT detectors

Capabilities

  • Field-of View Scanning system for imaging at speeds from 1 to 1800Hz
  • Scanning XY-stage for montage imaging
  • Stage top incubation system - 5% CO2,, temperature controlled, compatible with 35mm dish
  • Adaptive Focus Control
  • LightGate Imaging via pulsed WLL and HyD detectors helps remove reflected light as well as autofluorescence
  • Z-stack for 3-D rendering
  • Brightfield:
  • Transmission
  • Reflection
  • Fluorescence
  • Multi-color simultaneous acquition
  • Serial acquition
  • Time lapse
  • FRET
  • FRAP

Objectives

  • 63x Plan APO 1.4 NA oil (140um working distance)
  • 40x HC APO 1.3 NA oil (240um working distance)
  • 40x HCX PL APO  0.85 NA dry (210um working distance)
  • 20x HC PL APO 0.75 IMM (660um working distance)
  • 10x HC PL APO 0.4 NA dry (2.63mm working distance)

CALENDAR

For online scheduling navigate to iLabs for the core login page: https://ouhsc.corefacilities.org/

Minimum appointment time is 15 minutes and anything longer than 5 hours needs approval.

Any problems with iLab calendar read the help manuals below:

SAFE OPERATING PRACTICES  (read before using equipment)

SOP for Leica SP8              

Rates OUHSC External Industry
With operator assistance $65/ hour $135/ hour $175/ hour
Without operator assistance (must be trained) $35/ hour $100/ hour $110/ hour
Cancellation Charges (less than 24-hour notice) $35.00    

 LINKS