Leica SP8 Confocal
Lasers and Detectors
- White Light Laser system (470 to 670 nm) tunable in steps of 1 nm
- UV / 405 nm laser
- Violet / 442 nm laser
- 4 channels of Confocal Detection
- 2 Super Sensitive Spectral Hybrid detectors
- 2 cooled PMT detectors
Capabilities
- Field-of View Scanning system for imaging at speeds from 1 to 1800Hz
- Scanning XY-stage for montage imaging
- Stage top incubation system - 5% CO2,, temperature controlled, compatible with 35mm dish
- Adaptive Focus Control
- LightGate Imaging via pulsed WLL and HyD detectors helps remove reflected light as well as autofluorescence
- Z-stack for 3-D rendering
- Brightfield:
- Transmission
- Reflection
- Fluorescence
- Multi-color simultaneous acquition
- Serial acquition
- Time lapse
- FRET
- FRAP
Objectives
- 63x Plan APO 1.4 NA oil (140um working distance)
- 40x HC APO 1.3 NA oil (240um working distance)
- 40x HCX PL APO 0.85 NA dry (210um working distance)
- 20x HC PL APO 0.75 IMM (660um working distance)
- 10x HC PL APO 0.4 NA dry (2.63mm working distance)
CALENDAR
For online scheduling navigate to iLabs for the core login page: https://ouhsc.corefacilities.org/
Minimum appointment time is 15 minutes and anything longer than 5 hours needs approval.
Any problems with iLab calendar read the help manuals below:
SAFE OPERATING PRACTICES (read before using equipment)
Rates
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OUHSC
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External
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Industry
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With operator assistance
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$70/ hour
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$147/ hour
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$210/ hour
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Without operator assistance (must be trained)
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$40/ hour
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$80/ hour
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$120/ hour
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Cancellation Charges (less than 24-hour notice)
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$35.00
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LINKS