Proteomics or IP analysis

Thermo Tribrid Lumos Fusion Orbitrap, Thermo Quantis Triple Quadrupole

The mass spectrometry core facility at OUHSC provides services that include protein identification and protein quantification from a wide variety of complex sample types, from cell lysate, protein complexes and plasma. 

Our principal approach for protein analysis is the "bottom-up" analysis, where the protein samples are proteolytically digested, producing peptides that are signatures of the original proteins.

Protein quantification can be accomplished by:

  • Label free (no labeling, as many samples as wanted, most costly)
  • Chemical Labeling, TMT labeling (TMT allows to quantify and combine up to 16 samples into 1 experiment. Less mass spec time)
  • Metabolic Labeling, SILAC (stable isotope labeling of cell, combine up to 3 samples into 1 experiment)
  • Spiked Peptides, Targeted proteomics (need pure peptide for protein of interest)

We currently can perform open (unbiased) qualitative and quantitative analyses using high resolution, accurate mass data for high confidence identifications and quantitative reproducibility. This is the preferred tool for differential protein expression and biomarker discovery.

These experiments are performed on a Thermo Fusion Lumos Tribrid orbitrap (all proteomic analysis except targeted) coupled to ultra-performance nanoscale capillary liquid chromatography (LC/ESI/MS/MS) or on a Thermo Quantis triple quadrupole for targeted proteomics quantification.

Before submitting a sample for proteomic analysis, it is highly recommended that you consult with Dr. Virginie Sjoelund.

 

For online submission navigate to iLabs for the core login page: https://ouhsc.corefacilities.org/

Any problems with iLab read the help manuals below: